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(A) To separate blood into plasma and blood cells,the blood must be kept in a liquid state (prevented from clotting). $1$. Heparin and sodium oxalate

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Test tube containing calcium carbonate

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(A) To separate blood into plasma and blood cells,the blood must be kept in a liquid state (prevented from clotting). $1$. Heparin and sodium oxalate are anticoagulants that prevent blood clotting by removing or chelating calcium ions,which are essential for the coagulation cascade. $2$. $A$ sterile test tube is standard for collection. $3$. Calcium carbonate is a source of calcium ions. Adding calcium to blood promotes the coagulation process,which would cause the blood to clot,making it impossible to separate plasma and cells effectively. Therefore,a test tube containing calcium carbonate should not be used.

If you are to collect blood from a patient and store it in a test tube for the separation of blood cells and plasma,which of the following test tubes would you $NOT$ use?

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