What should a cloning vector NOT possess? | vedclass

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(D) cloning vector must possess an origin of replication $(ori)$ to allow autonomous replication. It must have a selectable marker to identify and eli

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More than one recognition site for a single restriction enzyme

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(D) cloning vector must possess an origin of replication $(ori)$ to allow autonomous replication. It must have a selectable marker to identify and eliminate non-transformants. It should have unique cloning sites for restriction enzymes to allow the insertion of foreign $DNA$. If a vector has more than one recognition site for a single restriction enzyme,it will lead to multiple fragments of the vector upon digestion,which complicates the gene cloning process. Therefore,it should not possess multiple recognition sites for the same restriction enzyme.

What should a cloning vector $NOT$ possess?

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