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(A) The cloning vector $pBR322$ is a widely used plasmid vector in genetic engineering.To facilitate the cloning process,it is essential that the vect

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(A) The cloning vector $pBR322$ is a widely used plasmid vector in genetic engineering.To facilitate the cloning process,it is essential that the vector contains unique recognition sites for various restriction enzymes.If a restriction enzyme had multiple recognition sites within the vector,the plasmid would be cleaved into multiple fragments upon digestion,which would make the insertion of the foreign $DNA$ segment impossible or highly inefficient.Therefore,$pBR322$ is engineered to have only $1$ (one) unique recognition site for each of the specific restriction enzymes used for cloning,such as $EcoRI$,$BamHI$,$HindIII$,etc.

How many recognition sites are present for each restriction enzyme in the cloning vector $pBR322$?

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In the nomenclature of the enzyme $Restriction$ $Endonuclease$,what does the Roman numeral indicate?

What was linked with the plasmid in the construction of the first $r-DNA$?

$A$ - $amp^R$ has recognition sites for $Pvu I$.
$R$ - $tet^R$ gene has no recognition site for $Hind III$.

$A$ plasmid $DNA$ and a linear $DNA$ (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis,the plasmid shows one $DNA$ band while the linear $DNA$ shows two fragments. Explain.

Restriction enzymes are:

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