If a foreign DNA fragment is inserted into th | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(D) The plasmid $pBR322$ contains two antibiotic resistance genes: $amp^R$ (ampicillin resistance) and $tet^R$ (tetracycline resistance).The $BamHI$ r

Vedclass · Accepted Answer

Medium containing tetracycline antibiotic

Vedclass · Answer

(D) The plasmid $pBR322$ contains two antibiotic resistance genes: $amp^R$ (ampicillin resistance) and $tet^R$ (tetracycline resistance).The $BamHI$ restriction site is located within the $tet^R$ gene.When a foreign $DNA$ fragment is inserted into the $BamHI$ site,it causes insertional inactivation of the $tet^R$ gene.As a result,the recombinant $DNA$ molecule loses its resistance to tetracycline.Therefore,the recombinants will not grow in a medium containing tetracycline antibiotic.

If a foreign $DNA$ fragment is inserted into the $BamHI$ site of $pBR322$ to create $r-DNA$,in which medium will the recombinants not grow?

Similar Questions

Hind $II$ always cuts $DNA$ molecules at a particular site called recognition sequence and it consists of : (in $bp$)

Which enzyme is responsible for joining two fragments of $DNA$?

Which of the following is commonly used to introduce foreign $DNA$ into cereal plants?

Which restriction enzyme produces blunt ends upon cleavage?

What is the correct nomenclature for restriction enzyme-$I$, obtained from $Bacillus$ $amyloliquefaciens$ $H$?

Vedclass Test Series

Exam Paper Generator

Online Exam Module