Select the correct sequence for PCR (Polymera | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(C) $PCR$ (Polymerase Chain Reaction) involves three main steps in each cycle:$1$. Denaturation: The double-stranded $DNA$ is heated to a high tempera

Vedclass · Accepted Answer

Denaturation $\rightarrow$ Annealing $\rightarrow$ Extension

Vedclass · Answer

(C) $PCR$ (Polymerase Chain Reaction) involves three main steps in each cycle:$1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature (approx. $94-98^{\circ}C$) to separate the strands.$2$. Annealing: The temperature is lowered (approx. $50-65^{\circ}C$) to allow primers to bind to the complementary sequences on the single-stranded $DNA$.$3$. Extension: The temperature is adjusted (approx. $72^{\circ}C$) for the $Taq$ polymerase to synthesize the new $DNA$ strand by adding nucleotides.Therefore,the correct sequence is Denaturation $\rightarrow$ Annealing $\rightarrow$ Extension.

Select the correct sequence for $PCR$ (Polymerase Chain Reaction).

Similar Questions

During the process of gene amplification using $PCR$,if very high temperature is not maintained in the beginning,then which of the following steps of $PCR$ will be affected first?

What is the process of elution?

The method involving the entry of high-velocity gold microparticles coated with $DNA$ into plant cells is known as:

If $r-DNA$ containing the $amp^R$ gene is transferred into $E. coli$ cells,what will the normal $E. coli$ cells be transformed into?

$RNA$ is removed by the treatment with

Vedclass Test Series

Exam Paper Generator

Online Exam Module