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(C) The figure illustrates the action of a restriction endonuclease on both vector $DNA$ and foreign $DNA$ to produce complementary sticky ends. Speci

Vedclass · Accepted Answer

$EcoR\,I$

Vedclass · Answer

(C) The figure illustrates the action of a restriction endonuclease on both vector $DNA$ and foreign $DNA$ to produce complementary sticky ends. Specifically,the recognition sequence shown for the enzyme is $5'-GAATTC-3'$ and $3'-CTTAAG-5'$,which is the specific recognition site for the restriction enzyme $EcoR\,I$. $EcoR\,I$ cuts the $DNA$ between the bases $G$ and $A$,resulting in the formation of sticky ends that facilitate the ligation of the foreign $DNA$ into the vector $DNA$.

$(a)$ Restriction endonuclease	$(i)$ Joins the $DNA$ fragments
$(b)$ Restriction exonuclease	$(ii)$ Extends primers on genomic $DNA$ template
$(c)$ $DNA$ ligase	$(iii)$ Cuts $DNA$ at specific positions
$(d)$ $Taq$ polymerase	$(iv)$ Removes nucleotides from the ends of $DNA$

Which enzyme is required for the process shown in the figure below?

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