(D) The cloning vector $pBR322$ is a widely used plasmid in biotechnology. Based on the standard map of $pBR322$,the restriction sites located on the

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(D) The cloning vector $pBR322$ is a widely used plasmid in biotechnology. Based on the standard map of $pBR322$,the restriction sites located on the ampicillin resistance gene $(amp^R)$ are $Pst I$ and $Pvu I$. Specifically,in the standard diagram,'$P$' corresponds to the $Pst I$ restriction site and '$Q$' corresponds to the $Pvu I$ restriction site. Therefore,the correct representation is $Pst I$ and $Pvu I$.

Class 12 Biology Biotechnology Principals and Process Mix Examples - Biotechnology Principals and Process

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In the given figure of the cloning vector $pBR322$,what do '$P$' and '$Q$' represent respectively?

A
$Pvu II$ and $Pst I$
B
$Pst I$ and $EcoRI$
C
$Cla I$ and $Pvu II$
D
$Pst I$ and $Pvu I$

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Class 12 Questions

Class 12

Biology Questions

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Biotechnology Principals and Process

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Mix Examples - Biotechnology Principals and Process

An antibiotic resistance gene in a vector helps in the selection of:

Medium

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$A$ - Transformation is a procedure through which a piece of $DNA$ is introduced in a host bacterium.
$R$ - With the help of $PCR$,thousands of $DNA$ copies are formed.

Medium

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Match the columns:
Column-$I$ Column-$II$
$(a)$ Ethidium Bromide $(i)$ Agrobacterium
$(b)$ Ti plasmid $(ii)$ Tetracycline
$(c)$ $PvuI$ $(iii)$ Gel electrophoresis
$(iv)$ Ampicillin

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Match the organism with its use in biotechnology:
$(a)$ Bacillus thuringiensis $(i)$ Cloning vector
$(b)$ Thermus aquaticus $(ii)$ Construction of first rDNA molecule
$(c)$ Agrobacterium tumefaciens $(iii)$ $DNA$ polymerase
$(d)$ Salmonella typhimurium $(iv)$ Cry proteins

$(a)\quad (b)\quad (c)\quad (d)$

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Identify the figure given below.

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Column-$I$	Column-$II$
$(a)$ Ethidium Bromide	$(i)$ Agrobacterium
$(b)$ Ti plasmid	$(ii)$ Tetracycline
$(c)$ $PvuI$	$(iii)$ Gel electrophoresis
	$(iv)$ Ampicillin

$(a)$ Bacillus thuringiensis	$(i)$ Cloning vector
$(b)$ Thermus aquaticus	$(ii)$ Construction of first rDNA molecule
$(c)$ Agrobacterium tumefaciens	$(iii)$ $DNA$ polymerase
$(d)$ Salmonella typhimurium	$(iv)$ Cry proteins

In the given figure of the cloning vector $pBR322$,what do '$P$' and '$Q$' represent respectively?

Similar Questions

An antibiotic resistance gene in a vector helps in the selection of:

$A$ - Transformation is a procedure through which a piece of $DNA$ is introduced in a host bacterium.$R$ - With the help of $PCR$,thousands of $DNA$ copies are formed.

Match the columns:Column-$I$Column-$II$$(a)$ Ethidium Bromide$(i)$ Agrobacterium$(b)$ Ti plasmid$(ii)$ Tetracycline$(c)$ $PvuI$$(iii)$ Gel electrophoresis$(iv)$ Ampicillin

Match the organism with its use in biotechnology:$(a)$ Bacillus thuringiensis$(i)$ Cloning vector$(b)$ Thermus aquaticus$(ii)$ Construction of first rDNA molecule$(c)$ Agrobacterium tumefaciens$(iii)$ $DNA$ polymerase$(d)$ Salmonella typhimurium$(iv)$ Cry proteins$(a)\quad (b)\quad (c)\quad (d)$

Identify the figure given below.

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$A$ - Transformation is a procedure through which a piece of $DNA$ is introduced in a host bacterium.
$R$ - With the help of $PCR$,thousands of $DNA$ copies are formed.

Match the columns:
Column-$I$ Column-$II$
$(a)$ Ethidium Bromide $(i)$ Agrobacterium
$(b)$ Ti plasmid $(ii)$ Tetracycline
$(c)$ $PvuI$ $(iii)$ Gel electrophoresis
$(iv)$ Ampicillin