Which bond is formed by DNA ligase to join a | vedclass

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Question

(C) $DNA$ ligase is an enzyme that acts as a 'molecular glue'.It catalyzes the formation of a phosphodiester bond between the $3'-OH$ end of one $DNA$

Vedclass · Accepted Answer

Phosphodiester bond

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(C) $DNA$ ligase is an enzyme that acts as a 'molecular glue'.It catalyzes the formation of a phosphodiester bond between the $3'-OH$ end of one $DNA$ fragment and the $5'-PO_4$ end of another $DNA$ fragment.This process is essential for sealing nicks in the sugar-phosphate backbone of $DNA$ during recombinant $DNA$ technology,thereby joining the foreign $DNA$ insert into the plasmid vector.

Which bond is formed by $DNA$ ligase to join a plasmid and a foreign $DNA$ fragment?

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