(B) The $PCR$ (Polymerase Chain Reaction) technique was invented by Kary Mullis in $1983$. This technique allows for the amplification of a specific $

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(B) The $PCR$ (Polymerase Chain Reaction) technique was invented by Kary Mullis in $1983$. This technique allows for the amplification of a specific $DNA$ segment in vitro. The provided information regarding antibiotic resistance markers in $E. coli$ is relevant to cloning vectors but is not related to the invention of $PCR$.