$A$ : $Pst I$ generates single-stranded overhanging stretches in $DNA$ after digestion that facilitate ligation.
$R$ : These short extensions can form hydrogen bonds with their complementary counterparts,which are then sealed by $DNA$ ligase to form phosphodiester bonds.
$R$ : These short extensions can form hydrogen bonds with their complementary counterparts,which are then sealed by $DNA$ ligase to form phosphodiester bonds.
- AAssertion and Reason both are correct and Reason is the correct explanation of Assertion.
- BAssertion and Reason both are correct but Reason is not the correct explanation of Assertion.
- CAssertion is correct,but Reason is incorrect.
- DBoth Assertion and Reason are incorrect.
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Statement-$I$: Thermostable $\text{DNA}$ polymerase is isolated from $\text{Thermus aquaticus}$.
Statement-$II$: $\text{Taq}$ $\text{DNA}$ polymerase is inactive at high temperature $(72^{\circ}\text{C})$.
Statement-$II$: $\text{Taq}$ $\text{DNA}$ polymerase is inactive at high temperature $(72^{\circ}\text{C})$.
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View SolutionThe key tools required for the recombinant $DNA$ technology are:
$I.$ Enzymes
$II.$ $RNA$ primer
$III.$ $DNA$ probe
$IV.$ Vectors
$V.$ Host organisms
Select the correct option.
$I.$ Enzymes
$II.$ $RNA$ primer
$III.$ $DNA$ probe
$IV.$ Vectors
$V.$ Host organisms
Select the correct option.
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View SolutionFrom where is agarose gel derived?
Consider the following statements:
$I.$ $A$ soil-inhabiting plant bacterium,$Agrobacterium \; tumefaciens$,a pathogen of several dicot plants,is able to transfer a piece of $DNA$ known as $T-DNA$.
$II.$ The $T-DNA$ causes tumours.
$III.$ Tumour formation is induced by $Ti-plasmid$.
Which of the statements given above are correct?
$I.$ $A$ soil-inhabiting plant bacterium,$Agrobacterium \; tumefaciens$,a pathogen of several dicot plants,is able to transfer a piece of $DNA$ known as $T-DNA$.
$II.$ The $T-DNA$ causes tumours.
$III.$ Tumour formation is induced by $Ti-plasmid$.
Which of the statements given above are correct?
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