$PCR$ is used to:

$Escherichia$ $coli$ $(E. coli)$ is widely used in biological research because:

Which one of the following pairs is not correctly matched?

Match List-$I$ with List-$II$:

List-$I$	List-$II$
$A$. Genetically modified organism	$I$. Agrobacterium tumefaciens
$B$. $DNA$ polymerase	$II$. Bt cotton
$C$. Ti plasmid	$III$. Thermus aquaticus
$D$. pBR322	$IV$. Escherichia coli

Choose the correct answer from the options given below:

Given below are two statements: one is labelled as Assertion $(A)$ and the other is labelled as Reason $(R)$.
Assertion $(A):$
Polymerase chain reaction is used in $DNA$ amplification.
Reason $(R):$
The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements,choose the correct answer from the options given below:

Match the following columns:

Column-$I$	Column-$II$
$(a)$ $EFB$	$(1)$ Recognition sequence
$(b)$ Biotechnology	$(2)$ Molecular scissors
$(c)$ Restriction enzyme	$(3)$ Definition of biotechnology
$(d)$ Palindromic sequence	$(4)$ Test tube baby