In the purification process of recombinant $DNA$ technology,what does the addition of chilled ethanol precipitate?

From the following tools/techniques of genetic engineering,identify those which are required for cloning a bacterial gene in animal cells and choose the correct option:
$I$. Endonuclease $II$. Ligase
$III$. $A. tumefaciens$ $IV$. Microinjection
$V$. Gene gun $VI$. Lysozyme
$VII$. Cellulase $VIII$. Electrophoresis

In a mixture,$DNA$ fragments are separated by

How many types of primers are required for the amplification of a gene in $PCR$?

$A$: To extract $RNA$ from tomato cells,it must first be treated with cellulase,protease,and then deoxyribonuclease.
$R$: Deoxyribonuclease will digest $DNA$ while $RNA$ will be intact.

$I.$ In the process of recombinant $DNA$ technology,after several treatments,the purified $DNA$ is precipitated by adding chilled ethanol.
$II.$ The bacterial,plant,or animal cell is broken down by enzymes to release $DNA$,along with $RNA$,proteins,polysaccharides,and lipids.
Choose the correct option for the above statements.