Which of the following are considered second-generation vaccines?

  • A
    Smallpox vaccine
  • B
    $D.P.T.$ vaccine
  • C
    Hepatitis $B$ vaccine
  • D
    Polio vaccine

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$III.$ $\alpha-1$-antitrypsin is used to treat $\text{PKU}$.
$IV.$ $\text{ELISA}$ serves the purpose of early diagnosis.
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Human insulin is being commercially produced from a transgenic species of

In the earlier times,the insulin used for diabetic patients was:

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