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(B) mutation is defined as a permanent alteration in the $DNA$ sequence that makes up a gene. The smallest possible change that can result in a mutati

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$A$ change in a single nucleotide

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(B) mutation is defined as a permanent alteration in the $DNA$ sequence that makes up a gene. The smallest possible change that can result in a mutation is a point mutation,which involves a change in a single nucleotide base pair within the $DNA$ sequence. This can lead to a change in the corresponding amino acid or have no effect,depending on the nature of the change.

The minimum requirement for a mutation is a change in:

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