Which of the following restriction enzymes used in recombinant $DNA$ technology produces staggered cuts (sticky ends) in $DNA$?

Given below are two statements: one is labelled as Assertion $(A)$ and the other is labelled as Reason $(R)$.
Assertion $(A):$
Polymerase chain reaction is used in $DNA$ amplification.
Reason $(R):$
The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements,choose the correct answer from the options given below:

Match the Column-$I$ with Column-$II$:

Column-$I$	Column-$II$
$(a)$ $K$.$C$. Mehta	$(i)$ Fluid mosaic model
$(b)$ $P$. Maheshwari	$(ii)$ First recombinant plasmid
$(c)$ Cohen and Boyer	$(iii)$ Haploid culture
$(d)$ Singer and Nicolson	$(iv)$ Rust disease

Match the following columns:

Column $I$	Column $II$
$A$. Recombinant $DNA$	$1$. Sea weeds
$B$. Gel electrophoresis	$2$. $DNA$ staining
$C$. Ethidium bromide	$3$. Plasmid $DNA$ that has incorporated human $DNA$
$D$. Agarose	$4$. Process by which $DNA$ fragments are separated based on their size

Match the following items in List-$I$ with List-$II$:

List-$I$	List-$II$
$(a)$ Gel electrophoresis	$(1)$ Amplification of gene of interest
$(b)$ $PCR$	$(2)$ Separation of $DNA$ fragments
$(c)$ Bioreactor	$(3)$ Plasmid
$(d)$ Cloning vector	$(4)$ Production of desired product

Identify '$U$','$V$' and '$W$' in the given figure of $pBR322$.