PCR is a three-step process based on temperat | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(A) $PCR$ (Polymerase Chain Reaction) involves three main steps:$1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature (approx.

Vedclass · Accepted Answer

Denaturation,Annealing,Extension

Vedclass · Answer

(A) $PCR$ (Polymerase Chain Reaction) involves three main steps:$1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature (approx. $94-98^{\circ}C$) to separate the strands.$2$. Annealing: The temperature is lowered (approx. $50-65^{\circ}C$) to allow primers to bind to the single-stranded $DNA$ templates.$3$. Extension: The temperature is adjusted (approx. $72^{\circ}C$) for the $Taq$ $DNA$ polymerase to synthesize the new $DNA$ strand by adding nucleotides.Therefore,the correct sequence is Denaturation,Annealing,and Extension.

$PCR$ is a three-step process based on temperature,which follows the sequence of ........

Similar Questions

Which one of the following equipments is essentially required for growing microbes on a large scale for the industrial production of enzymes?

What is required for the expression of a foreign gene?

What are bioreactors?

Taking examples under each category,discuss upstream and downstream processing.

The technique of bombarding plant cells with high velocity microparticles of gold or tungsten,coated with $DNA$,is known as:

Vedclass Test Series

Exam Paper Generator

Online Exam Module