What is required for the functioning of a clo | vedclass

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(D) cloning vector is a small piece of $DNA$ that can be stably maintained in an organism and into which a foreign $DNA$ fragment can be inserted for

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Origin of replication $(ori)$

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(D) cloning vector is a small piece of $DNA$ that can be stably maintained in an organism and into which a foreign $DNA$ fragment can be inserted for cloning purposes. For a vector to function,it must possess an $ori$ ($Origin$ of replication) sequence. This sequence is responsible for initiating replication within the host cell. Without an $ori$ site,the vector cannot replicate,and the foreign $DNA$ linked to it will not be multiplied.

What is required for the functioning of a cloning vector $DNA$?

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