Which of the following cell structures is non-living?

Which of the following layers is present nearest to the plasma membrane in a plant cell?

Assertion $A$: The middle lamella is present between the primary walls of two adjacent plant cells.
Reason $R$: Two adjacent cells maintain cytoplasmic contact through plasmodesmata.
Which option is correct for Assertion $A$ and Reason $R$?

$A$ student placed two cells in the same solution in two different containers. The observation was given in the table:

Container	Observation
$1$	Cell burst
$2$	Cell does not change its shape

Which structure maintains the shape of the cell present in container $2$ and provides the most significant difference between the two cells?

In plants,the cytoplasm of two adjacent cells is connected by .......... .

The cell wall is ........