The transfer of the streptomycin resistance gene from $Nicotiana$ $tabacum$ to $Nicotiana$ $sylvestris$ was achieved through which method?

Modern biotechnology includes:

Match the following columns and select the correct option.

Column-$I$	Column-$II$
$(a)$ $Bt$ cotton	$(i)$ Gene therapy
$(b)$ Adenosine deaminase deficiency	$(ii)$ Cellular defence
$(c)$ $RNAi$	$(iii)$ Detection of $HIV$ infection
$(d)$ $PCR$	$(iv)$ Bacillus thuringiensis

Identify the mismatched pair.

Statement $I$: $\text{RNA}$ interference $\text{(RNAi)}$ takes place in all eukaryotic organisms as a method of cellular defense.
Statement $II$: $Bt$ toxin protein is converted into an active form of toxin due to the alkaline $pH$ of the gut of an insect.

Match the columns:

Column-$I$	Column-$II$
$(1)$ Golden Rice	$(a)$ Bud insects
$(2)$ Bt toxin	$(b)$ Vitamin-$A$
$(3)$ Biopiracy	$(c)$ Cry-protein
$(4)$ Bt tobacco	$(d)$ Bio-source without permission and use of bioreactor