What is a callus?

$A:$ In plant tissue culture,the nutrients in the medium decrease.
$R:$ In this method,the biomass of cells or tissues increases.

Why are plants obtained by protoplast culture called somatic hybrids?

What is the part of the plant used in tissue culture called?

Arrange the following steps of somatic hybridisation in a correct sequence.
$A$. Digestion of cell walls.
$B$. Isolation of naked protoplasts.
$C$. Fusion of protoplasts to get hybrid protoplast.
$D$. Isolation of single cells from two different varieties of plants.
$E$. Growing of hybrid protoplast to form a new plant.
Choose the correct answer from the options given below:

Which statement is correct regarding $Pomato$?