At which temperature do enzymes lose their native conformation?

In most plant cells,most enzymes have their optimum activity at: (in $^\circ C$)

Fumarase enzyme belongs to which class?

Enzymes are proteins. Proteins are long chains of amino acids linked to each other by peptide bonds. Amino acids have many functional groups in their structure. Many of these functional groups are ionisable. As they are weak acids and bases in chemical nature,this ionisation is influenced by the $pH$ of the solution. For many enzymes,activity is influenced by the surrounding $pH$. This is depicted in the curve below. Explain briefly.

Select the correct match for $X$ and $Y$:

Column $X$	Column $Y$
$(1)$ Transferases	$(P)$ Maltase
$(2)$ Hydrolases	$(Q)$ Hexokinase
$(3)$ Lyases	$(R)$ Fructose isomerase
$(4)$ Isomerases	$(S)$ Aldolase

Non-competitive inhibitors alter: