A mixture of fragmented DNA was electrophores | vedclass

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(B) The reasons for not observing $DNA$ bands are as follows:$(i)$ The $DNA$ sample loaded on the gel may have been contaminated with nucleases (exonu

Vedclass · Accepted Answer

The $DNA$ was degraded by nucleases or moved out of the gel due to incorrect electrode orientation.

Vedclass · Answer

(B) The reasons for not observing $DNA$ bands are as follows:$(i)$ The $DNA$ sample loaded on the gel may have been contaminated with nucleases (exonucleases,endonucleases,or both) and completely degraded.$(ii)$ The electrodes were placed in the opposite orientation in the gel assembly,meaning the anode was placed towards the wells where the $DNA$ sample was loaded. Since $DNA$ molecules are negatively charged,they move towards the anode and thus move out of the gel instead of migrating through the gel matrix.$(iii)$ Ethidium bromide was not added at all,or it was not added in a sufficient concentration,making the $DNA$ invisible under $UV$ light.

$A$ mixture of fragmented $DNA$ was electrophoresed in an agarose gel. After staining the gel with ethidium bromide,no $DNA$ bands were observed. What could be the reason?

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