About gene gun method:I. This method is also | vedclass

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(D) The gene gun method,also known as the biolistic technique,is a direct gene transfer method used primarily for plants.Statement $I$ is correct: It

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$I, II,$ and $III$

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(D) The gene gun method,also known as the biolistic technique,is a direct gene transfer method used primarily for plants.Statement $I$ is correct: It is indeed called the biolistic technique.Statement $II$ is correct: In this process,cells are bombarded with high-velocity micro-particles of gold or tungsten coated with the target $DNA$.Statement $III$ is correct: This technique has been successfully used to transform important crop plants such as maize,rice,and wheat.Therefore,all three statements are correct.

$(a)$ Restriction endonuclease	$(i)$ Joins the $DNA$ fragments
$(b)$ Restriction exonuclease	$(ii)$ Extends primers on genomic $DNA$ template
$(c)$ $DNA$ ligase	$(iii)$ Cuts $DNA$ at specific positions
$(d)$ $Taq$ polymerase	$(iv)$ Removes nucleotides from the ends of $DNA$

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