Assertion : Restriction enzymes cut the stran | vedclass

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(C) Restriction enzymes,a type of endonuclease,function by inspecting the length of a $DNA$ sequence. Once they find a specific recognition sequence,t

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If the Assertion is correct but Reason is incorrect.

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(C) Restriction enzymes,a type of endonuclease,function by inspecting the length of a $DNA$ sequence. Once they find a specific recognition sequence,they bind to it and cut each of the two strands of the double helix at specific points,leaving single-stranded portions at the ends. This results in overhanging stretches called sticky ends. These are named so because they form hydrogen bonds with their complementary counterparts; i.e.,they can join similar complementary ends of $DNA$ fragments from other sources with the help of the enzyme $DNA$ ligase. Therefore,the stickiness of the ends facilitates the action of the enzyme $DNA$ ligase,not $DNA$ polymerase. Thus,the Assertion is correct,but the Reason is incorrect.

Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.
Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase.

Similar Questions

Recombinant $DNA$ can be obtained by cutting pro-$DNA$ using:

Recombinant $DNA$ is achieved by cleaving the pro-$DNA$ by

Restriction endonucleases are:

Which of the following restriction enzymes produces blunt ends in $DNA$?

The function of a restriction endonuclease enzyme is to:

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Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase.