Provide definitions/explanations for: Cloning,Elution,and Insertional Inactivation.
(N/A) $1$. Cloning: The process of creating multiple copies of a specific segment of foreign $DNA$ by inserting it into a suitable vector and allowing it to replicate within a host organism.
$2$. Elution: The process of extracting the separated $DNA$ fragments from the agarose gel after gel electrophoresis. The specific $DNA$ band is cut out from the gel and the $DNA$ is purified from the gel piece.
$3$. Insertional Inactivation: $A$ method used to identify recombinant colonies. When a foreign gene is inserted into a selectable marker gene (e.g.,an antibiotic resistance gene) present in the plasmid,the marker gene becomes inactivated. This loss of function allows for the differentiation between recombinant and non-recombinant cells.
$2$. Elution: The process of extracting the separated $DNA$ fragments from the agarose gel after gel electrophoresis. The specific $DNA$ band is cut out from the gel and the $DNA$ is purified from the gel piece.
$3$. Insertional Inactivation: $A$ method used to identify recombinant colonies. When a foreign gene is inserted into a selectable marker gene (e.g.,an antibiotic resistance gene) present in the plasmid,the marker gene becomes inactivated. This loss of function allows for the differentiation between recombinant and non-recombinant cells.
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