Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
(N/A) The tumor-inducing $(Ti)$ plasmid of $Agrobacterium$ $tumefaciens$ has been modified into a cloning vector. This modified plasmid is no longer pathogenic to plants but retains the ability to use its natural mechanism to deliver genes of interest into a variety of plant cells. This process is widely used in genetic engineering to create transgenic plants.
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To isolate $DNA$ from fungal cells,bacterial cells,and plant cells,the enzymes required are respectively:
Which enzyme is known as molecular scissors?
Medium
View Solution$T_{i}$ plasmids used in genetic engineering are obtained from:
Easy
View Solution$I.$ $Ori$ also controls the copy number of the linked $DNA$.
$II.$ If a foreign $DNA$ ligates at the $BamHI$ site of the tetracycline resistance gene in the vector $pBR322$,the recombinant plasmid loses the tetracycline resistance due to the insertion of foreign $DNA$.
Choose the correct option regarding the above statements.
$II.$ If a foreign $DNA$ ligates at the $BamHI$ site of the tetracycline resistance gene in the vector $pBR322$,the recombinant plasmid loses the tetracycline resistance due to the insertion of foreign $DNA$.
Choose the correct option regarding the above statements.
Medium
View Solution$A$: Restriction enzymes belong to a larger class of enzymes called nucleases.
$R$: Each restriction enzyme recognizes a specific palindromic nucleotide sequence in the $DNA$.
$R$: Each restriction enzyme recognizes a specific palindromic nucleotide sequence in the $DNA$.
Medium
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