During in vitro synthesis of DNA,a researcher | vedclass

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(B) During $DNA$ synthesis,the $3'$-$OH$ group on the deoxyribose sugar is essential for the formation of a phosphodiester bond with the incoming nucl

Vedclass · Accepted Answer

Synthesis of $DNA$ will stop immediately.

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(B) During $DNA$ synthesis,the $3'$-$OH$ group on the deoxyribose sugar is essential for the formation of a phosphodiester bond with the incoming nucleotide. $2', 3'$-dideoxy cytidine triphosphate lacks the $3'$-$OH$ group. Therefore,once this molecule is incorporated into the growing $DNA$ chain,no further nucleotides can be added,leading to the termination of $DNA$ synthesis.

During in vitro synthesis of $DNA$,a researcher used $2', 3'$-dideoxy cytidine triphosphate as raw nucleotide in place of $2'$-deoxy cytidine. What would be the consequence?

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