For plant tissue culture,which of the following is required?

The nutrient medium for tissue culture should have:
$I.$ Sucrose
$II.$ Inorganic Salts
$III.$ Amino acids
$IV.$ Vitamins

Read the following statements:
$I.$ Colostrum is recommended for newborns because it is rich in antigens.
$II.$ Chikungunya is caused by a Gram-negative bacterium.
$III.$ Tissue culture has proved useful in obtaining virus-free plants.
$IV.$ Beer is manufactured by the distillation of fermented grapes.
How many of the statement$(s)$ is/are correct?

Somaclonal variation appears in

In which of the following are somaclonal variations observed?

What is meant by a dormant callus?