What are the full forms of $ELISA$ and $PCR$?

Agarose gel electrophoresis is used to separate $DNA$ fragments. The $DNA$ fragments separate due to . . . . . . .

Select the correct statement.

Explain how the synthesis of a gene of interest is performed using $PCR$.

Which of the following steps are involved in the process of recombinant biotechnology? Arrange in correct order.
$I.$ Extraction of the desired gene product
$II.$ Amplification of the gene of interest
$III.$ Isolation of a desired $DNA$ fragment
$IV.$ Ligation of the $DNA$ fragment into a vector
$V.$ Insertion of recombinant $DNA$ into the host
Correct order is:

$PCR$ (Polymerase Chain Reaction) is used for the diagnosis of which of the following diseases?