The gene of interest was cloned at the Sal I | vedclass

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(A) The plasmid $pBR322$ contains two antibiotic resistance genes: $amp^R$ (ampicillin resistance) and $tet^R$ (tetracycline resistance).When a gene o

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Tetracycline

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(A) The plasmid $pBR322$ contains two antibiotic resistance genes: $amp^R$ (ampicillin resistance) and $tet^R$ (tetracycline resistance).When a gene of interest is inserted (cloned) at the $Sal I$ restriction site,it disrupts the $tet^R$ gene.This process is known as insertional inactivation.As a result,the recombinant plasmid loses its resistance to tetracycline and becomes susceptible to it,while it remains resistant to ampicillin.

The gene of interest was cloned at the $Sal I$ site in $pBR322$. The recombinant plasmid will exhibit susceptibility to:

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