Having become an expert on gel electrophoresi | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(A) $DNA$ molecules are negatively charged due to the phosphate backbone. In gel electrophoresis,$DNA$ fragments are loaded into wells near the negati

Vedclass · Accepted Answer

Near the positive electrode,farthest away from the wells

Vedclass · Answer

(A) $DNA$ molecules are negatively charged due to the phosphate backbone. In gel electrophoresis,$DNA$ fragments are loaded into wells near the negative electrode (cathode). When an electric field is applied,the $DNA$ fragments migrate towards the positive electrode (anode). The gel matrix acts as a molecular sieve,where smaller fragments move faster and travel further through the pores of the gel compared to larger fragments. Therefore,the smallest $DNA$ segments are found farthest from the wells,near the positive electrode.

Having become an expert on gel electrophoresis,you are asked to examine a gel for a colleague. Where would you find the smallest segment of $DNA$?

Similar Questions

The preparatory phase before fermentation is known as:

An artificial process of infecting cells with naked viral $DNA$ is:

What is downstream processing?

Select the correct statements from the following:

In $pBR322$,foreign $DNA$ is inserted using the restriction enzyme $Cla\, I$. If this recombinant plasmid is introduced into bacteria,which of the following will occur regarding growth on antibiotic-containing media?

Vedclass Test Series

Exam Paper Generator

Online Exam Module