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(B) Henking $(1891)$ was the first to trace a specific nuclear structure during spermatogenesis in a few insects. He observed that $50 \%$ of the sper

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$XX - XO$ method

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(B) Henking $(1891)$ was the first to trace a specific nuclear structure during spermatogenesis in a few insects. He observed that $50 \%$ of the sperms received this specific nuclear structure,which he named the $X$-body,while the other $50 \%$ did not. Later,it was discovered that the $X$-body was actually a chromosome,now called the $X$-chromosome. This type of sex determination,where males have one $X$-chromosome and one missing chromosome (denoted as $O$),is known as the $XX - XO$ type of sex determination. In this system,females are homogametic $(XX)$,and males are heterogametic $(XO)$.

Henking observed that in a few male insects,$50 \%$ of the sperm received a specific nuclear structure known as $X$-body,whereas the other $50 \%$ of sperms did not receive it. This method of sex determination was:

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