In a confocal microscope,the specimen is illu | vedclass

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(B) confocal microscope uses a point light source,typically a laser beam,to illuminate the specimen. This allows for the collection of optical section

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Laser beam

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(B) confocal microscope uses a point light source,typically a laser beam,to illuminate the specimen. This allows for the collection of optical sections from different depths of the sample,which are then reconstructed to form a three-dimensional image. Unlike conventional fluorescence microscopy,it uses a spatial pinhole to block out-of-focus light,resulting in higher resolution and contrast.

In a confocal microscope,the specimen is illuminated by:

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