In genetic engineering,two bacteria are very | vedclass

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(A) In genetic engineering,$Escherichia$ $coli$ $(E. coli)$ is widely used as a cloning host due to its well-understood genetics and rapid growth. $Ag

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Escherichia coli and Agrobacterium tumefaciens

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(A) In genetic engineering,$Escherichia$ $coli$ $(E. coli)$ is widely used as a cloning host due to its well-understood genetics and rapid growth. $Agrobacterium$ $tumefaciens$ is a soil bacterium that naturally transfers a segment of its $DNA$ ($T$-$DNA$) into plant cells,making it an essential tool for plant genetic engineering and the creation of transgenic plants. Therefore,these two bacteria are considered the most useful in genetic engineering applications.

Column-$I$	Column-$II$
$(a)$ Ligase	$(i)$ It removes nucleotides from $DNA$ ends.
$(b)$ Endonuclease	$(ii)$ Connects parts of $DNA$.
$(c)$ Exonuclease	$(iii)$ Removes phosphates from $DNA$.
$(d)$ $DNA$ Polymerase	$(iv)$ It produces sticky ends in $DNA$.
	$(v)$ Useful for repeated amplification.

In genetic engineering,two bacteria are very useful. They are ......

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