In Meselson and Stahl's experiments,heavy DNA | vedclass

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(D) In the experiment conducted by Meselson and Stahl to prove semi-conservative $DNA$ replication,they used $E. coli$ grown in a medium containing $^

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$CsCl$ gradient

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(D) In the experiment conducted by Meselson and Stahl to prove semi-conservative $DNA$ replication,they used $E. coli$ grown in a medium containing $^{15}N$ (a heavy isotope of nitrogen) as the only nitrogen source for many generations. This resulted in the incorporation of $^{15}N$ into the newly synthesized $DNA$ (heavy $DNA$). Subsequently,these cells were transferred to a medium with normal $^{14}NH_4Cl$. To distinguish between heavy $DNA$ $(^{15}N-DNA)$ and normal $DNA$ $(^{14}N-DNA)$,they performed density gradient centrifugation using a $CsCl$ (caesium chloride) gradient. The $CsCl$ gradient allows $DNA$ molecules to separate based on their buoyant density,where heavier molecules settle at a different position compared to lighter ones.

In Meselson and Stahl's experiments,heavy $DNA$ was distinguished from normal $DNA$ by centrifugation in

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