In the presence of a chromogenic substrate,re | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(B) In recombinant $DNA$ technology,the process of insertional inactivation is used to distinguish recombinant colonies from non-recombinant ones. The

Vedclass · Accepted Answer

Colourless colonies

Vedclass · Answer

(B) In recombinant $DNA$ technology,the process of insertional inactivation is used to distinguish recombinant colonies from non-recombinant ones. The enzyme $\beta$-galactosidase is encoded by the $lacZ$ gene. When a foreign $DNA$ fragment is inserted into the $lacZ$ gene,the enzyme becomes inactivated. In the presence of a chromogenic substrate,non-recombinant bacteria (containing the functional $lacZ$ gene) produce blue-coloured colonies. However,recombinant bacteria (where the $lacZ$ gene is interrupted) fail to produce the enzyme and therefore appear as colourless colonies.

In the presence of a chromogenic substrate,recombinant bacteria give:

Similar Questions

In $pBR322$,foreign $DNA$ is inserted using the restriction enzyme $Cla\, I$. If this recombinant plasmid is introduced into bacteria,which of the following will occur regarding growth on antibiotic-containing media?

In gel electrophoresis,the separated $DNA$ fragments are visualized after staining the $DNA$ with $...A...$ followed by exposure to $...B...$. Here $A$ and $B$ refer to:

Transformation is a process in which ..........

Which of the following diagnostic tools allows the detection of very low concentration of bacteria or viruses by amplifying their nucleic acid?

What is required for the expression of a foreign gene?

Vedclass Test Series

Exam Paper Generator

Online Exam Module