In the technique of DNA fingerprinting,digest | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(A) The technique of $DNA$ fingerprinting involves several steps: isolation of $DNA$,digestion of $DNA$ by restriction endonucleases,separation of $DN

Vedclass · Accepted Answer

Electrophoresis

Vedclass · Answer

(A) The technique of $DNA$ fingerprinting involves several steps: isolation of $DNA$,digestion of $DNA$ by restriction endonucleases,separation of $DNA$ fragments by gel electrophoresis,transferring (blotting) of separated $DNA$ fragments to synthetic membranes (like nitrocellulose or nylon),hybridization using labeled $VNTR$ probe,and detection of hybridized $DNA$ fragments by autoradiography.Therefore,after the digestion of $DNA$ by restriction enzymes,the next step is the separation of these fragments based on their size using gel electrophoresis.

In the technique of $DNA$ fingerprinting,digestion of $DNA$ is followed by:

Similar Questions

Dr. Lalji Singh obtained a radioactive $DNA$ probe from the $Y$ chromosome of:

Which of the following steps is not involved in $DNA$ fingerprinting?

'There is a paternity dispute for a child'. Which technique can solve the problem? Discuss the principle involved.

In Northern blotting,$RNAs$ are separated by gel electrophoresis and the $RNA$ bands are transferred onto a membrane of:

In which of the following steps in the $DNA$ fingerprinting technique are labelled $VNTR$ probes used?

Vedclass Test Series

Exam Paper Generator

Online Exam Module