Intact chloroplasts from green leaves can be | vedclass

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(D) To isolate intact chloroplasts from green leaves,an isotonic or hypertonic solution is required to maintain the structural integrity of the organe

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Sugar solution

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(D) To isolate intact chloroplasts from green leaves,an isotonic or hypertonic solution is required to maintain the structural integrity of the organelle. Acetone,ethanol,and alcohol are organic solvents that dissolve the lipid bilayer of the chloroplast membrane,causing the organelle to rupture. $A$ sugar solution (such as sucrose) provides an osmotic environment that prevents the chloroplast from bursting,allowing for the isolation of intact organelles.

Intact chloroplasts from green leaves can be isolated by using:

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