Significance of heat shock method in bacteria | vedclass

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Question

(C) The heat shock method is a technique used in genetic engineering to introduce recombinant $DNA$ into bacterial cells (like $E. coli$).Bacteria are

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Uptake of $DNA$ through transient pores in the bacterial cell wall

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(C) The heat shock method is a technique used in genetic engineering to introduce recombinant $DNA$ into bacterial cells (like $E. coli$).Bacteria are generally not capable of taking up foreign $DNA$ directly from their environment.To make them 'competent',they are treated with a divalent cation (like $Ca^{2+}$),which increases the efficiency with which $DNA$ enters the bacterium through pores in its cell wall.During the heat shock process,the cells are incubated with recombinant $DNA$ on ice,then placed briefly at $42^{\circ}C$ (heat shock),and finally put back on ice.This sudden temperature change creates transient pores in the bacterial cell wall,allowing the recombinant $DNA$ to enter the cell.

Significance of heat shock method in bacterial transformation is to facilitate

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