The significance of the heat shock method in | vedclass

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(C) Bacterial cells do not take up recombinant $DNA$ easily because $DNA$ is a hydrophilic molecule and cannot pass through cell membranes. To force b

Vedclass · Accepted Answer

Uptake of $DNA$ through transient pores in the bacterial cell wall

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(C) Bacterial cells do not take up recombinant $DNA$ easily because $DNA$ is a hydrophilic molecule and cannot pass through cell membranes. To force bacteria to take up the $DNA$,they are first treated with a specific concentration of a divalent cation,such as calcium $(Ca^{2+})$,which increases the efficiency with which $DNA$ enters the bacterium through pores in its cell wall. Recombinant $DNA$ can then be forced into such cells by incubating the cells with recombinant $DNA$ on ice,followed by placing them briefly at $42^{\circ}C$ (heat shock),and then putting them back on ice. This heat shock enables the bacteria to take up the recombinant $DNA$ through transient pores created in the cell wall.

The significance of the heat shock method in bacterial transformation is that it facilitates:

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