The genetic defect-adenosine deaminase $(ADA)$ deficiency may be cured permanently by

In $1990$,which therapy was given to a four-year-old girl suffering from adenosine deaminase $(ADA)$ deficiency?

Match the following columns.

Column-$I$	Column-$II$
$(A)$ Gene therapy	$(1)$ Effort to fix a functional gene
$(B)$ Humulin	$(2)$ $A$ single-stranded $DNA$ or $RNA$ tagged with a radioactive molecule
$(C)$ Probe	$(3)$ Diagnostic test
$(D)$ $ELISA$	$(4)$ Diabetes

Which of the following statements are false?
$I$. Insulin for curing diabetes,used to be extracted from the pancreas of slaughtered pig and cattle.
$II$. Animal insulin is slightly different from human insulin.
$III$. Animal insulin causes some undesirable side effects such as allergy.
$IV$. Bacteria cannot be made to synthesise insulin from its gene because of the presence of introns.
Choose the correct option.

Correct chronological order of the steps occurring during gene therapy are
$I$. Lymphocytes are obtained from the patients
$II$. Lymphocytes are transferred to the culture dishes
$III$. Lymphocytes are transfected with the normal $ADA$ genes
$IV$. The transfected cells are returned to the patients
The chronological order should be

Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
$(i)$ Synthesis of gene $(DNA)$ for human insulin artificially.
(ii) Culturing recombinant $E. coli$ in bioreactors.
(iii) Purification of humulin.
(iv) Insertion of human insulin gene into plasmid.
$(v)$ Introduction of recombinant plasmid into $E. coli$.
(vi) Extraction of recombinant gene product from $E. coli$.