The primer extension during PCR cycle require | vedclass

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Question

(A) The $PCR$ (Polymerase Chain Reaction) process consists of three main steps: denaturation,annealing,and extension.$1$. Denaturation occurs at $94-9

Vedclass · Accepted Answer

$70-75^{\circ} C$

Vedclass · Answer

(A) The $PCR$ (Polymerase Chain Reaction) process consists of three main steps: denaturation,annealing,and extension.$1$. Denaturation occurs at $94-98^{\circ} C$.$2$. Annealing occurs at $50-65^{\circ} C$.$3$. Primer extension is performed by the $Taq$ polymerase enzyme,which is heat-stable and functions optimally at a temperature range of $70-75^{\circ} C$. Therefore,the correct option is $A$.

The primer extension during $PCR$ cycle requires the temperature range of . . . . . . .

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