Tissue culture technique can produce an infinite number of new plants from a small parental tissue. The economic importance of the technique is in raising

Which method is used to develop new plant species in botanical gardens?

Which of the following is commonly used as a carbon source in the nutrient medium for plant tissue culture?

Arrange the following steps of somatic hybridisation in a correct sequence.
$A$. Digestion of cell walls.
$B$. Isolation of naked protoplasts.
$C$. Fusion of protoplasts to get hybrid protoplast.
$D$. Isolation of single cells from two different varieties of plants.
$E$. Growing of hybrid protoplast to form a new plant.
Choose the correct answer from the options given below:

Which enzyme is useful for obtaining plant protoplasts?

What are the various components of the medium used for the in vitro propagation of explants?