Tissue culture technique requires

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(D) Tissue culture requires a specialized laboratory environment to ensure successful growth and prevent contamination.Complete sterilization of all g

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(D) Tissue culture requires a specialized laboratory environment to ensure successful growth and prevent contamination.Complete sterilization of all glassware (flasks,petri dishes,test tubes) and instruments is essential to eliminate microbes and other living organisms that could interfere with the culture.The nutrient medium used for the growth of explants is adjusted to a specific $pH$ range,typically between $5.0$ and $6.0$,to support optimal cellular activity.Furthermore,the cultures are maintained under strictly controlled environmental conditions,including light and a temperature range of $18^{\circ}C$ to $25^{\circ}C$.

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