What are the physical barriers of a cell in the protoplast fusion experiment? How are these barriers overcome?

(N/A) The primary physical barrier in a plant cell during protoplast fusion experiments is the rigid cell wall.
To overcome this barrier,the plant tissue is treated with specific cell wall-degrading enzymes,primarily cellulase and pectinase.
These enzymes digest the cellulose and pectin components of the cell wall,respectively,thereby releasing the naked protoplast,which is bounded only by the plasma membrane.