What modification is done on the Ti plasmid o | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(A) The tumor-inducing $(Ti)$ plasmid of $Agrobacterium$ $tumefaciens$ is modified by removing the $T-DNA$ region,which is responsible for tumor forma

Vedclass · Accepted Answer

Removal of the $T-DNA$ region

Vedclass · Answer

(A) The tumor-inducing $(Ti)$ plasmid of $Agrobacterium$ $tumefaciens$ is modified by removing the $T-DNA$ region,which is responsible for tumor formation in plants.This process is known as 'disarming' the plasmid.By removing the $T-DNA$ and replacing it with the gene of interest,the plasmid becomes a non-pathogenic cloning vector.It retains the ability to transfer the desired gene into plant cells using its natural $vir$ $(virulence)$ gene machinery.

What modification is done on the $Ti$ plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?

Similar Questions

Which of the following would not be used in preparing recombinant $DNA$?

The first discovered restriction endonuclease that always cuts $DNA$ molecules at a particular point by recognizing a specific sequence of six base pairs is:

$A$ plasmid

In bacteria,a plasmid is .........

Which restriction enzyme produces blunt ends upon cleavage?

Vedclass Test Series

Exam Paper Generator

Online Exam Module