Which of the following is important in genetic engineering?

From which organism is the $Taq$ polymerase enzyme isolated?

Plasmids are used as vectors because ........

Which of the following enzyme$(s)$ are $\text{NOT}$ essential for gene cloning?
$A.$ Restriction enzymes
$B.$ $\text{DNA}$ ligase
$C.$ $\text{DNA}$ mutase
$D.$ $\text{DNA}$ recombinase
$E.$ $\text{DNA}$ polymerase
Choose the correct answer from the options given below:

Select the option that excludes characteristics applicable to plasmids:
$(a)$. Circular $DNA$
$(b)$. Linear $DNA$
$(c)$. Present in all bacteria
$(d)$. Contain essential genes
$(e)$. Extra chromosomal self-replicating

$A$: Restriction enzymes belong to a larger class of enzymes called nucleases.
$R$: Each restriction enzyme recognizes a specific palindromic nucleotide sequence in the $DNA$.