Which of the following is produced on an industrial scale using biotechnology?

Which country's company first produced human insulin?

The human protein named $\alpha-1$-antitrypsin, obtained from transgenic animals, is used for the treatment of . . . . . . .

The main challenge for the production of insulin using $rDNA$ techniques was getting insulin into its matured form using $........$ bonds.

Which protein production was successfully introduced in $E. coli$?

Which of the following is/are correct about Adenosine Deaminase $(ADA)$ deficiency?
$I$. In the absence of adenosine deaminase enzyme,purine metabolism is disturbed and $T$-lymphocytes fail to function.
$II$. $ADA$ deficiency is caused by the deletion of the gene for $ADA$.
$III$. In some cases,it can be cured by bone marrow transplantation and enzyme replacement therapy. But in both approaches,the patients are not completely cured.
$IV$. For permanent cure,genes isolated from the bone marrow cells producing $ADA$ at early embryonic stages can be a possible cure.
Which of the above statements are correct?