Which of the following is used in tissue culture?

Read the following statements:
$I.$ Colostrum is recommended for newborns because it is rich in antigens.
$II.$ Chikungunya is caused by a Gram-negative bacterium.
$III.$ Tissue culture has proved useful in obtaining virus-free plants.
$IV.$ Beer is manufactured by the distillation of fermented grapes.
How many of the statement$(s)$ is/are correct?

Which of the following is $NOT$ an application of callus and suspension culture?

In crop improvement programmes,virus-free clones can be obtained through

Arrange the following steps of somatic hybridisation in a correct sequence.
$A$. Digestion of cell walls.
$B$. Isolation of naked protoplasts.
$C$. Fusion of protoplasts to get hybrid protoplast.
$D$. Isolation of single cells from two different varieties of plants.
$E$. Growing of hybrid protoplast to form a new plant.
Choose the correct answer from the options given below:

In tissue culture,which part of the plant is taken to develop a new healthy plant from a diseased plant?