Which of the following steps is/are catalysed by $Taq$ polymerase in a $PCR$?

Identify the correct sequence of steps in the Polymerase Chain Reaction $(PCR)$.

In this technique,large quantities of biotechnological products such as antibiotics,vaccines,and enzymes are produced by maintaining and growing only the desired microbes/eukaryotic cells in a sterile environment.

Which of the following diagrams is correct for primer annealing in $PCR$?

The method involving the entry of high-velocity gold microparticles coated with $DNA$ into plant cells is known as:

The steps involved in the Southern blot test are as follows:
$I.$ $X-ray$ film
$II.$ Electrophoresis
$III.$ Digestion with restriction enzyme
$IV.$ Ethidium bromide
$V.$ Radioactive probe
Choose the option having the correct sequential order of these events.